3D bioprinting and biofabrication

Pre-homework questions:

  • From an ethical and moral standpoint, list pros and cons of working with hiPSCs as opposed to embryonic stem cells?

  • If we are to use organoid models as surrogates for in vivo tissues, what type of functional attributes would be critical to add/engineer into such organoid systems?

  • Can you think of any limitations in current organoid and or organ on a chip systems in recapitulating human biology?

  • If you were to theorize an experiment about improving current organoid models, which aspects of the microenvironment would you consider adding beyond chemical?

  • In the tissue engineering paradigm, what properties are important to provide from a scaffold and bioreactor?

The homework component will include:

  1. Read prescribed articles and answer questions.

  1. Live streamed treatment of EHTs with student input of which drugs to use, reasoning behind selection of drugs (what do each of them do?), concentrations to use and if incremental dosing is required?