Aromata-supporting material
Freeze-Dried Cell-Free Biosynthesis
Cell-Free(CF) Bio-production
Fig. 1. Components of a cell-free protein synthesis reaction: (extract, supplements, and a DNA template) with the key reactions that occur when they are combined.
Melinek et al., “Toward a Roadmap for Cell-Free Synthesis in Bioprocessing.”
Freeze-Dried Cell-Free(FD-CR) reactions
Fig. 2. BioBitsTM kits: Freeze-dried educational kits. (A) FD-CF demonstrations require only the addition of water to the supplied reactions and incubation for 1 to 20 hours at 25° to 37°C for observation and analysis by students. In contrast, traditional biology experiments require substantial time, resources, and specialized equipment. (B) With the DNA template and any substrate molecules provided with the FD-CF reaction, the students just have to add water to run a number of bioscience activities and demonstrations.
FD-CF Expression of Aroma Compounds
Fig. 3. Fragrance-generating enzymes as olfactory outputs. (A) Using FD-CF reactions, we manufactured enzymes that can generate various smells from the Saccharomyces cerevisiae acetyltransferase ATF1. (B) Production of fragrance molecules after substrate addition to overnight FD-CF reactions of ATF1, as detected by headspace GC-MS. Values represent averages, and error bars represent SDs of n = 3 biological replicates.
Microfluidic Bioreactor
Cell-Free Microfluidic Bioreactor
Figure. 4.Serpentine channel microfluidic bioreactor design for cell-free production of biotherapeutics.
Abeille et al., “Continuous Microcarrier-Based Cell Culture in a Benchtop Microfluidic Bioreactor.”
Microfluidic Bioreactor
Fig. 5.A 3D-printed microfluidic bioreactor for organ-on-chip cell culture.
Wearable Olfactory CHI
miniaturized aroma release unit
Fig. 6. Modular scent delivery holders, A) one-piece structure, and B) multi-part decorative structure. C) Design explorations of the scent release mechanism based on 1) Angle between the piezo and the tube. 2) Length and shape of the tube, 3) Assembly of multiple scent release, 4) Clip-on accessories or embeddings in jewelry and piercings.
Olfactory Interface Placements
Fig. 7. Prototypes that we used for the user study. 1) "Glasses" prototype, 2) "Nose" prototype, and 3) Olfactory necklace. Participants wear the PCB board and battery on their left ear for both on-face designs while hooking the holder at the back part of the cloth for the necklace.
User Experience Results
Fig. 8. Likert Scale for 1 = Extremely Inappropriate or Extremely Uncomfortable and 9 = Extremely Appropriate or Extremely Comfort- able. Error bars correspond to ±1 S.D.
Fig. 9. "Moist" - humidity felt on the face, "Smell" - intensity of the smell, "Burst" - visual spray, "Sound" - emitted when a burst is released. Error bars correspond to ±1 S.D. The wearers smelled the fragrance significantly more than the observers for all the prototypes